In-situ construction of enzyme-copper nucleotide composite for efficient chemo-enzymatic cascade reaction

• Published in: Applied catalysis. A, General Vol. 608; p. 117899
• Main Authors: Wu, Xiaoling, Liu, Shuli, Xiong, Jun, Chen, Bin, Zong, Min-Hua, Yang, Ji-Guo, Lou, Wen-Yong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.11.2020; Elsevier Science SA
• Subjects: Aminophenol; Analgesics; Aqueous solutions; Biomanufacturing; Biosensors; Cascade chemical reactions; Cascade reaction; Catalysis; Catalysts; Catalytic activity; Chemical engineering; Chemical reactions; Chemical synthesis; Chemo-enzymatic catalysis; Copper; Enzyme-metal nucleotide composite; Enzymes; In-situ assembly; Lipase; Nucleotides; Cascade reaction; Chemo-enzymatic catalysis; Biomanufacturing; In-situ assembly; Enzyme-metal nucleotide composite
• ISSN: 0926-860X; 1873-3875
• DOI: 10.1016/j.apcata.2020.117899

[Display omitted] •A lipase-copper hybrid catalyst was developed via in-situ assembly strategy.•Copper nucleotide composite ensured uniformed distribution of copper nanoparticles.•Hybrid catalyst featured high enzymatic and copper catalytic activity.•Hybrid catalyst enabled highly efficient production of p-AP in cascade reaction. The integration of chemical catalyst and biocatalyst is of great interest due to the accompanying high designability of chemo-enzymatic cascade reactions for sustainable catalytic processes in chemical engineering. Here, a facile strategy is proposed by preparation of a copper ions coordinated cytidine monophosphate (CMP) encapsulated lipase composite via in-situ assembly in aqueous solution which followed by reduction to obtain an enzyme-copper hybrid catalyst. The as-synthesized enzyme-copper nucleotide composite exhibited both high enzymatic activity (6.05 U/mg) and copper-catalytic activity (with reaction rate constant of 8.85 min−1) in the chemo-enzymatic cascade reaction for hydrolysis of p-nitrophenyl butyrate (p-NPB) and generation of p-aminophenol (p-AP), which is a pharmaceutical intermediate for production of analgesics and antipyretics. This study thus sheds light on fabrication of hybrid catalyst under facile conditions, exhibiting potential in development of biosensors, industrial catalysis and biomanufacturing.