Distribution of wheat germ agglutinin binding sites on normal human and guinea pig bone marrow cells: an ultrastructural histochemical study

The wheat germ agglutinin-gold labeled ovomucoid (WGA-Ovo-G) method has been employed in the ultrastructural localization of WGA surface receptor sites on gluteraldehyde-fixed normal human and guinea pig bone marrow cells. The number of gold particles per micron of cell surface were counted and data...

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Bibliographic Details
Published inThe Anatomical record Vol. 195; no. 4; p. 641
Main Author Ackerman, G A
Format Journal Article
LanguageEnglish
Published United States 01.12.1979
Subjects
Animals
Binding Sites, Antibody
Bone Marrow - ultrastructure
Gold Colloid, Radioactive
Guinea Pigs
Histocytochemistry
Humans
Lectins
Ovomucin
Plant Lectins
Staining and Labeling
Triticum
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Summary:The wheat germ agglutinin-gold labeled ovomucoid (WGA-Ovo-G) method has been employed in the ultrastructural localization of WGA surface receptor sites on gluteraldehyde-fixed normal human and guinea pig bone marrow cells. The number of gold particles per micron of cell surface were counted and data subjected to statistical analysis. WGA labeling of the bone marrow cells was found to be quite heavy and evenly distributed along the cell surface. Only macrophages, in both species, and a number of lymphocytes in the guinea pig exhibited limited WGA labeling. Comparison of morphologically similar cell types in human and guinea pig marrow indicated that WGA receptor sites were more abundant on the guinea pig hemic cells. WGA surface labeling was found to be not only related to cell type and to animal species but, in some instances, varied with the stage of maturation of a given cell line; viz., neutrophilic cell series in both man and guinea pig and erythrocytic cell series in the guinea pig.
ISSN:0003-276X
1097-0185
DOI:10.1002/ar.1091950406