Raman spectroscopic study of serum albumins: an effect of proton- and γ-irradiation

Raman spectroscopy was applied to analyse structural changes in serum albumins (bovine serum albumin, BSA; human serum albumin, HSA) following proton and γ‐irradiation (0.5, 5 and 50 Gy). Characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation. S...

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Published inJournal of Raman spectroscopy Vol. 38; no. 12; pp. 1646 - 1655
Main Authors Synytsya, Alla, Alexa, Petr, de Boer, Jorrit, Loewe, Markus, Moosburger, Martin, Würkner, Michael, Volka, Karel
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 01.12.2007
Subjects
proton and γ-irradiation
Raman spectroscopy
serum albumins
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Summary:Raman spectroscopy was applied to analyse structural changes in serum albumins (bovine serum albumin, BSA; human serum albumin, HSA) following proton and γ‐irradiation (0.5, 5 and 50 Gy). Characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation. Significant damage of HSA/BSA was observed only at the highest dose (50 Gy). Raman spectra confirmed radiation‐induced denaturation, destruction of helical structures and aggregation of serum albumins. The differences in the dose‐dependent effects of proton and γ‐radiation on studied proteins are discussed. Copyright © 2007 John Wiley & Sons, Ltd.
Bibliography:istex:F035F29B3F4DDA638224DD6095408629B14FD55D
ArticleID:JRS1884
ark:/67375/WNG-BM6W9G7B-4
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0377-0486
1097-4555
DOI:10.1002/jrs.1884