Observing the deoxy myoglobin and hemogobin signals from rat myocardium in situ

• Published in: FEBS letters Vol. 434; no. 3; pp. 309 - 312
• Main Authors: Tran, Tuan-Khanh, Kreutzer, Ulrike, Jue, Thomas
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 04.09.1998
• Subjects: DSS, dimethylsilapentane sulfonate; Hb, hemoglobin; Hemoglobin; Mb, myoglobin; Myocardium; Myoglobin; Nuclear magnetic resonance; pO2, partial pressure of oxygen; Myoglobin; Hb, hemoglobin; Mb, myoglobin; pO 2, partial pressure of oxygen; Hemoglobin; Myocardium; Nuclear magnetic resonance; DSS, dimethylsilapentane sulfonate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)01001-1

1H NMR proximal histidyl N δH signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO 2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O 2, the erythrocyte Hb is partially desaturated and yields the α and β proximal histidyl N δH signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO 2.