Furanoligularenone, an eremophilane from Ligularia fischeri, inhibits the LPS-induced production of nitric oxide and prostaglandin E2 in macrophage RAW264.7 cells

• Published in: Planta medica Vol. 68; no. 2; p. 101
• Main Authors: Hwang, Bang Yeon, Lee, Jeong-Hyung, Koo, Tae Hyeon, Kim, Hang Sub, Hong, Young Soo, Ro, Jai Seup, Lee, Kyong Soon, Lee, Jung Joon
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2002
• Subjects: Animals; Asteraceae; Cell Line; Cell Survival - drug effects; Cyclooxygenase 2; Dinoprost - antagonists & inhibitors; Dinoprost - biosynthesis; Dose-Response Relationship, Drug; Gene Expression Regulation, Enzymologic - drug effects; Isoenzymes - antagonists & inhibitors; Isoenzymes - genetics; Isoenzymes - metabolism; Lipopolysaccharides - pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Mice; Molecular Structure; Naphthalenes - chemistry; Naphthalenes - isolation & purification; Naphthalenes - pharmacology; NF-kappa B - metabolism; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type II; Plant Extracts - pharmacology; Prostaglandin-Endoperoxide Synthases - genetics; Prostaglandin-Endoperoxide Synthases - metabolism; RNA, Messenger - antagonists & inhibitors; RNA, Messenger - metabolism; Sesquiterpenes - chemistry; Sesquiterpenes - isolation & purification; Sesquiterpenes - pharmacology
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-2002-20250

Furanoligularenone (1), a known eremophilane, was identified from Ligularia fischeri (Compositae) together with 3-oxo-8alpha-hydroxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide (2) and 3-oxo-8alpha-methoxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide (3), by its potent inhibition of LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 cells. Compound 1 also suppressed the expression of iNOS and COX-2 mRNA and protein in a dose-dependent manner. Taken together, compound 1 inhibits the production of NO and PGE2 at the transcription of iNOS and COX-2 genes, and would be responsible for the anti-inflammatory activities of the Ligularia fischeri.