The probiotic, Leuconostoc mesenteroides, inhibits Listeria monocytogenes biofilm formation

• Published in: Journal of food safety Vol. 40; no. 2
• Main Authors: Shao, Xinhao, Fang, Kuili, Medina, Daniel, Wan, Jason, Lee, Jung‐Lim, Hong, Seok Hoon
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.04.2020; Blackwell Publishers Inc
• Subjects: Bacteria; Biofilms; Cell culture; Food contamination; Food irradiation; Food processing; Food safety; Lactic acid; Lactic acid bacteria; Leuconostoc mesenteroides; Listeria; Listeria monocytogenes; Probiotics; Sanitation; Serotypes; Species; Strains (organisms)
• ISSN: 0149-6085; 1745-4565
• DOI: 10.1111/jfs.12750

Listeria monocytogenes biofilm formation renders these cells highly resistant to current sanitation methods, and probiotics may be a promising approach to the efficient inhibition of Listeria biofilms. In the present study, three Leuconostoc mesenteroides strains of lactic acid bacteria isolated from kimchi were shown to be effective probiotics for inhibiting Listeria biofilm formation. Biofilms of two L. monocytogenes serotypes, 1/2a (ATCC15313) and 4b (ATCC19115), in dual‐species culture with each probiotic strain were decreased by more than 40‐fold as compared with single‐species Listeria biofilms; for instance, a reduction from 5.4 × 106 colony forming units (CFU)/cm2 L. monocytogenes ATCC19115 in single‐species biofilms to 1.1 × 105 CFU/cm2 in dual‐species biofilms. Most likely, one of the Leuconostoc strains, L. mesenteroides W51, led to the highest Listeria biofilm inhibition without affecting the growth of L. monocytogenes. The cell‐free supernatant from the L. mesenteroides W51 culture containing large protein molecules (>30 kDa) also inhibited Listeria biofilms. These data indicate that Leuconostoc probiotics can be used to repress L. monocytogenes biofilm contamination on surfaces at food processing facilities. Leuconostoc mesenteroides strains isolated from kimchi were shown to be effective probiotics for inhibiting Listeria monocytogenes biofilm formation more than 40‐fold. The cell‐free supernatant from the L. mesenteroides culture also decreased Listeria biofilms. This study indicates that kimchi probiotics can be applied to repress Listeria biofilm contamination on surfaces at food processing facilities.