Identification of disaccharides by gas chromatography-Fourier transform infrared spectroscopy

The use of GC-Fourier transform (FT) IR of methylsilyl ethers of disaccharides to positively identify disaccharides is reported for the first time. The conditions required to separate these high-molecular-mass compounds by GC, in the quantities required for FT-IR detection are reported. Trimethylsil...

Full description

Saved in:
Bibliographic Details
Published inJournal of Chromatography A Vol. 750; no. 1; pp. 311 - 316
Main Authors Veness, R.G., Evans, C.S.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 25.10.1996
Elsevier
Subjects
Analytical chemistry
Carbohydrates
Chemistry
Chromatographic methods and physical methods associated with chromatography
Derivatization, GC
Disaccharides
Exact sciences and technology
Gas chromatographic methods
Disaccharides
Carbohydrates
Derivatization, GC
Infrared spectrometry
Gas chromatography
Fourier transformation
Chemical analysis
Coupled method
Silylation
Qualitative analysis
Glycoside
Derivatization
Disaccharide
Online AccessGet full text

Cover

More Information
Summary:The use of GC-Fourier transform (FT) IR of methylsilyl ethers of disaccharides to positively identify disaccharides is reported for the first time. The conditions required to separate these high-molecular-mass compounds by GC, in the quantities required for FT-IR detection are reported. Trimethylsilyl ethers can be used successfully. The use of dimethylsilyl ethers is not recommended as their instability, under the required operating conditions, resulted in fragmentation and rearrangements.
ISSN:0021-9673
DOI:10.1016/0021-9673(96)00281-6