Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy

Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite i...

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Published inNature microbiology Vol. 1; no. 10; p. 16124
Main Authors Wakisaka, Yoshifumi, Suzuki, Yuta, Iwata, Osamu, Nakashima, Ayaka, Ito, Takuro, Hirose, Misa, Domon, Ryota, Sugawara, Mai, Tsumura, Norimichi, Watarai, Hiroshi, Shimobaba, Tomoyoshi, Suzuki, Kengo, Goda, Keisuke, Ozeki, Yasuyuki
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.08.2016
Subjects
Algae
Animals
Biomaterials
Euglena gracilis
Euglena gracilis - cytology
Euglena gracilis - metabolism
Labeling
Microscopy, Video - methods
Optical Imaging - methods
Scattering, Radiation
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Statistical analysis
Water - parasitology
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Summary:Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.
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ISSN:2058-5276
2058-5276
DOI:10.1038/NMICROBIOL.2016.124