Laser-diffusion measurements on δ-endotoxin crystals from Bacillus thuringiensis var. kurstaki HD1

Laser light scattering has been employed to investigate changes in the hydrodynamic properties of δ-endotoxin crystals of Bacillus thuringiensis var. kurstaki HD1. Crystals are polydisperse. Estimates of the mean hydrodynamic diameter agree with those obtained by light microscopy. Sodium dodecyl sul...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 840; no. 3; pp. 423 - 427
Main Authors Sattelle, D.B., Haniff, C.A., Thomas, W.E., Ellar, D.J.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 05.07.1985
Elsevier
North-Holland
Subjects
B. thuringiensis
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Laser light scattering
Microbiology
Morphology, structure, chemical composition
δ-Endotoxin structure
Laser light scattering
B. thuringiensis
δ-Endotoxin structure
Bacillales
Bacillus thuringiensis
Light scattering
Crystals
Bacteria
Phase contrast microscopy
Bacillaceae
Endotoxin
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Summary:Laser light scattering has been employed to investigate changes in the hydrodynamic properties of δ-endotoxin crystals of Bacillus thuringiensis var. kurstaki HD1. Crystals are polydisperse. Estimates of the mean hydrodynamic diameter agree with those obtained by light microscopy. Sodium dodecyl sulphate (SDS) at a final concentration of 1% results in swelling of the toxin crystals. Dithiothreitol-induced solubilization of the swollen crystals indicates the importance of disulphide bridges to the maintenance of the assembled crystal.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(85)90224-7