The use of methanol extract of Leptospira interrogans in complement fixation tests for leptospirosis

Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients...

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Bibliographic Details
Published inCanadian journal of microbiology Vol. 37; no. 6; p. 455
Main Authors Koury, M C, Cisalpino, E O, Rangel, H de A
Format Journal Article
LanguageEnglish
Published Canada 01.06.1991
Subjects
Agglutination Tests
Antigens, Bacterial - immunology
Complement Fixation Tests - methods
Humans
Leptospira - isolation & purification
Leptospirosis - diagnosis
Methanol
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Summary:Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of leptospirosis were tested by complement fixation tests and microscopic agglutination reactions. Agreement of 84% was found for those two reactions. Positive microscopic agglutination tests at a dilution 1:20-1:400 with negative complement fixation tests were observed in 5% of patients and negative microscopic agglutination with complement fixation tests in the range of 1:20-1:1280 were observed in 11% of the cases.
ISSN:0008-4166
DOI:10.1139/m91-075