Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data....

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Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 28; no. 4; pp. 689 - 693
Main Authors de Camargo, Camila, Vicentini, Marcia, Gobbi, Angelo, Martinez, Diego, Lima, Renato
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 01.04.2017
Subjects
CHEMISTRY, MULTIDISCIPLINARY
colorimetry
negatoscope
rapid test
human plasma
albumin
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Summary:We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%.
ISSN:0103-5053
1678-4790
DOI:10.21577/0103-5053.20160214