Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae

Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisola...

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Published inBio-protocol Vol. 12; no. 9; p. e4403
Main Authors Suzuki, Sho W, Emr, Scott D
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.05.2022
Bio-protocol LLC
Subjects
Biology
Methods
Vesicles
Recycling
Endosome
Immunoisolation
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Abstract Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
AbstractList Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( i.e. , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
Author Suzuki, Sho W
Emr, Scott D
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Immunoisolation
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Snippet Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are...
Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( i.e. , retromer, and Mvp1) are...
Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are...
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Title Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
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