Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae

Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisola...

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Bibliographic Details
Published inBio-protocol Vol. 12; no. 9; p. e4403
Main Authors Suzuki, Sho W, Emr, Scott D
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.05.2022
Bio-protocol LLC
Subjects
Biology
Methods
Vesicles
Recycling
Endosome
Immunoisolation
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Summary:Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes ( , retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast. This method is a powerful way to characterize endosomal recycling pathways.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4403