Peculiarities of receptor-channel complexes for inhibitory mediators in the membranes of lamprey spinal cord neurones

• Published in: Neuroscience letters Vol. 102; no. 1; p. 82
• Main Authors: Safronov, B V, Baev, K V, Batueva, I V, Rusin, K I, Suderevskaya, E I
• Format: Journal Article
• Language: English
• Published: Ireland 17.07.1989
• Subjects: Animals; Bicuculline - pharmacology; Fishes - physiology; gamma-Aminobutyric Acid - pharmacology; Glycine - pharmacology; In Vitro Techniques; Lampreys - physiology; Neural Inhibition - drug effects; Receptors, Neurotransmitter - drug effects; Receptors, Neurotransmitter - physiology; Spinal Cord - drug effects; Spinal Cord - physiology; Strychnine - pharmacology
• ISSN: 0304-3940
• DOI: 10.1016/0304-3940(89)90311-X

Effects of inhibitory mediators on the membranes of isolated lamprey spinal cord neurones were investigated by means of whole-cell recording and concentration clamp techniques. Glycine and gamma-aminobutyric acid (GABA) applications evoked desensitizing chloride currents. The concentrations at half-maximum effects were 16 microM for glycine- and 1.5 mM for GABA-activated currents. Ionic responses to applications of both amino acids demonstrated full cross-desensitization. Strychnine and bicuculline suppressed both glycine- and GABA-activated conductances to the same degree. We suggest that in the membranes of lamprey spinal cord neurones glycine and GABA act on the same receptor-channel complexes.