Resolution of diastereomeric phosphoramidate bridged unnatural oligonucleotides by micellar electrokinetic chromatography

Micellar electrokinetic chromatography was used to resolve diastereomers of oligonucleotides possessing several chiral phosphoramidate bridges. These materials were not resolved by conventional liquid chromatographic techniques. Parameters that were found to affect electrophoretic resolution include...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 636; no. 1; pp. 113 - 123
Main Authors Bevan, C.D., Mutton, I.M., Pipe, A.J.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 23.04.1993
Elsevier
Subjects
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:Micellar electrokinetic chromatography was used to resolve diastereomers of oligonucleotides possessing several chiral phosphoramidate bridges. These materials were not resolved by conventional liquid chromatographic techniques. Parameters that were found to affect electrophoretic resolution included the nature and concentration of surfactant, presence of organic modifiers such as propan-2-ol and urea, use of deuterium oxide-based buffers and temperature control. Practical optimization was achieved by employing Terabe's resolution principles.
ISSN:0021-9673
DOI:10.1016/0021-9673(93)80063-E