Application for proteomics analysis technology in studying animal-derived traditional Chinese medicine: A review

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 191; p. 113609
• Main Authors: Tang, Xue-mei, Guo, Jin-lin, Chen, Lu, Ho, Paul Chi-Lui
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.11.2020
• Subjects: 2-DE; iTRAQ; MELDI-TOF-MS; Proteomics; Traditional Chinese medicine; DDA; VA-Pro; CS-6; Animal TCM; Hsp90; MELDI-TOF-MS; 6−OHDA; HUVEC; PAEs; nano LC–MS/MS; DIA; 2D-DIGE; MALDI; Traditional Chinese medicine; ESI-MS; MDLC; 2-DE; IEF; SILAC; SWATH; MAPK; SDS-PAGE; CHMs; Proteomics; iTRAQ
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2020.113609

•The current main techniques of proteomics were summarized.•Applications of proteomics on animal - derived TCM research were first reviewed.•Existing problems and future direction on applications of proteomics on animal - derived TCM research were discussed. Different therapeutically active ingredients, from plants, animals, and mineral sources, are prescribed as traditional Chinese medicines (TCM). TCMs, from animal sources, are rich in proteins and peptides. Different advanced proteomics technologies, such as two-dimensional gel electrophoresis (2-DE), multi-dimensional liquid chromatography (MDLC), matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), and isobaric tags for relative and absolute quantitation (iTRAQ), have been applied to analyze TCMs, from animal sources. This paper reviews the common proteomic techniques for analyzing animal - derived TCMs. Various scientific studies have reported the application of proteomics for locating drug targets, identifying active components, and elucidating the mechanisms of action of animal - derived TCMs. However, these researches are still at the preliminary stage. This review has also discussed the existing challenges and future directions in this field of research.