Molecular Characterization and Physical Mapping of Two Classes of 5S rDNA in the Genomes of Gymnotus sylvius and G. inaequilabiatus (Gymnotiformes, Gymnotidae)

The nucleotide sequences of the 5S rRNA multigene family and their distribution across the karyotypes in 2 species of Gymnotiformes, genus Gymnotus (G. sylvius and G. inaequilabiatus) were investigated by means of fluorescence in situ hybridization (FISH). The results showed the existence of 2 disti...

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Published inCytogenetic and genome research Vol. 136; no. 2; pp. 131 - 137
Main Authors Scacchetti, P.C., Alves, J.C.P., Utsunomia, R., Claro, F.L., de Almeida Toledo, L.F., Oliveira, C., Foresti, F.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland S. Karger AG 01.01.2012
Subjects
Animals
Base Sequence
Chromosome Mapping - methods
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Electrophoresis, Agar Gel
Genome - genetics
Gymnotiformes - classification
Gymnotiformes - genetics
In Situ Hybridization, Fluorescence
Karyotype
Karyotyping
Molecular Sequence Data
Original Article
Polymerase Chain Reaction
RNA, Ribosomal, 5S - genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Species Specificity
Knife-fishes
Dispersed repetitive sequence family
Fish cytogenetics
Minor ribosomal genes
Comparative gene mapping
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Summary:The nucleotide sequences of the 5S rRNA multigene family and their distribution across the karyotypes in 2 species of Gymnotiformes, genus Gymnotus (G. sylvius and G. inaequilabiatus) were investigated by means of fluorescence in situ hybridization (FISH). The results showed the existence of 2 distinct classes of 5S rDNA sequences in both species: class I and class II. A high conservative pattern of the codifying region of the 5S rRNA gene was identified, contrasting with significant alterations detected in the nontranscribed spacer (NTS). The presence of TATA-like sequences along the NTS of both species was an expected occurrence, since such sequences have been associated with the regulation of the gene expression. FISH using 5S rDNA class I and class II probes revealed that both gene classes were collocated in the same chromosome pair in the genome of G. sylvius, while in that of G. inaequilabiatus, class II appeared more disperse than class I.
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ISSN:1424-8581
1424-859X
DOI:10.1159/000335658