The enzymic formation of dolichyl phosphate mannose from C-3 enantiomeric dolichyl phosphates

The racemic, optically inactive mixture of C-3 enantiomeric forms of C 95-dolichyl phosphate can be prepared chemically from fully unsaturated, di trans-polycis-C 95-prenol isolated from leaves of Sorbus suecica. Purified mannosyl transferase from yeast was capable of catalysing the mannosylation of...

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Published inBiochimica et biophysica acta Vol. 793; no. 2; pp. 187 - 192
Main Authors Chojnacki, Tadeusz, Palamarczyk, Grazyna, Jankowski, Wiesław, Krajewska-Rychlik, Izabella, Szkopińska, Anna, Vogtman, Tomasz
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.01.1984
Elsevier
North-Holland
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Dolichyl phosphate mannose
Enantiomer
Fundamental and applied biological sciences. Psychology
Lipids
Mannosyl transferase
Other biological molecules
Stereochemistry
Dolichyl phosphate mannose
Stereochemistry
Enantiomer
Mannosyl transferase
Enzymatic synthesis
Vegetals
Dicotyledones
Angiospermae
Rosaceae
Spermatophyta
Biosynthesis
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Summary:The racemic, optically inactive mixture of C-3 enantiomeric forms of C 95-dolichyl phosphate can be prepared chemically from fully unsaturated, di trans-polycis-C 95-prenol isolated from leaves of Sorbus suecica. Purified mannosyl transferase from yeast was capable of catalysing the mannosylation of over 75% of this 1:1 R/ S mixture, demonstrating that both enantiomers could function as acceptors. However, the R-form appears to accept mannose more slowly than the S-form. Both R- and S-forms of C 75-dolichyl phosphate and C 55-dolichyl phosphate prepared from the respective plant tri trans-poly cis-prenol were also active as lipid acceptors of mannose from GDPmannose.
ISSN:0005-2760
0006-3002
1879-145X
1878-2434
DOI:10.1016/0005-2760(84)90320-5