Metabolism of arachidonic acid involved in its eliciting activity in potato tuber

• Published in: Physiological and molecular plant pathology Vol. 41; no. 2; pp. 127 - 137
• Main Authors: Castoria, R., Fanelli, C., Fabbri, A.A., Passi, S.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 01.08.1992; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; AA; GC-MS; LAH; 5-S-HETE; EPA; 5-S-HPETE; HR; PL; Metabolic intermediate; Biochemical analysis; Enzyme; Coupled method; Solanum tuberosum; Biochemical compound; Polyunsaturated fatty acid; Lipids; Arachidonic acid; Elicitor; Metabolism; Fatty acids; Biological compound; Gas chromatography; Dicotyledones; Angiospermae; Tuber; Unsaturated fatty acid; Spermatophyta; Elicitation; Tuber plant; Solanaceae; Lipoxygenase; Mass spectrometry
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/0885-5765(92)90005-G

A 5-lipoxygenase from potato converts arachidonic acid (AA) to the unstable and reactive intermediate 5-S-hydroperoxyeicosatetraenoic acid (5-S-HPETE) which, at a concentration of 1 μg per tuber slice, induces phytoalexin accumulation to higher levels than those induced by AA at 20 μg per slice. However the tissue browning elicited by the 5-S-HPETE was lower than that elicited by AA, indicating that 5-S-HPETE may account for phytoalexin induction but not for the whole browning process. GC-MS analyses of lipid extracts of potato tuber slices treated with 1-[ 14C]AA and other polyunsaturated fatty acids showed no incorporation of these acids into potato lipid fractions.