Comparison of protein adsorption by anion-exchange interaction onto porous hollow-fiber membrane and gel bead-packed bed

• Published in: Journal of membrane science Vol. 117; no. 1; pp. 135 - 142
• Main Authors: Kubota, Noboru, Miura, Suguru, Saito, Kyoichi, Sugita, Kazuyuki, Watanabe, Kohei, Sugo, Takanobu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21.08.1996; Elsevier
• Subjects: Anion-exchange porous membrane; Biological and medical sciences; Biotechnology; Bovine serum albumin; Fundamental and applied biological sciences. Psychology; Hollow-fiber membrane; Membrane chromatography; Methods. Procedures. Technologies; Others; Radiation-induced graft polymerization; Various methods and equipments; Bovine serum albumin; Membrane chromatography; Hollow-fiber membrane; Radiation-induced graft polymerization; Anion-exchange porous membrane; Proteins; Binding capacity; Purification; Adsorption; Hollow fiber; Method; Anion exchange membrane; Comparative study; Mass transfer; Ion exchange chromatography
• ISSN: 0376-7388; 1873-3123
• DOI: 10.1016/0376-7388(96)00054-3

Protein collection performance by anion-exchange interaction was compared between a single porous hollow-fiber membrane and a gel bead-packed bed under identical conditions from a viewpoint of flow resistance and breakthrough behavior. At a space velocity (SV) of 100 h −1, an ethanolamino-group-containing porous hollow-fiber membrane (EA-C fiber) exhibited the same dynamic binding capacity for bovine serum albumin as a bed charged with diethylaminoethyl-group-containing gel beads (DEAE-G bed). Because mass transfer of the protein was enhanced by convective flow of the protein solution through the pores of the EA-C fiber, the EA-C fiber exhibited a higher productivity than the DEAE-G bed over SV = 100 h −1.