Regeneration and analysis of plants from stolon segments of Trifolium repens (white clover)

• Published in: Plant science (Limerick) Vol. 61; no. 1; pp. 119 - 125
• Main Authors: Bond, James E., Webb, K.Judith
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1989; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; In vitro culture; Plant physiology and development; plant regeneration; somaclonal variation; stolon segments; Tissue cultures, protoplasts; Trifolium repens; white clover; somaclonal variation; 2,4-D; stolon segments; white clover; plant regeneration; Trifolium repens; NAA; Culture medium; Callus; Stolon; Tissue culture; Environmental factor; Somaclonal variation; Regeneration; Leguminosae; Dicotyledones; Morphology; Angiospermae; Plant growth substance; Spermatophyta; Fodder crop
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/0168-9452(89)90125-8

Stolon segments from a highly regenerative line of Trifolium repens cv. Grasslands Huia provide a source of tissue for reliable and efficient de novo regeneration of plants. Media containing 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA) and kinetin induced the production of callus, shoots and/or embryoid-like structures. In the absence of 2,4-D, stolon explants formed multiple shoots alone at a high frequency. The regenerative capacity of the explants was directly related to their proximity to the apical meristem: segments from short internodes or those segments which were closest to the apex in longer internodes were the most responsive. A maximum of 72% of segments produced shoots after 14 days in culture. Regenerated plants were transferred to soil after 46 days in culture. One-hundred and twenty-nine regenerated plants were examined for morphological and biochemical variation. Twelve of these regenerants were also analysed cytologically.