ABri peptide aggregation quantification by fluorescamine and alpha imager assay

Familial British Dementia (FBD) is the early-onset autosomal dominant disorders characterized by cognitive impairment. The ABri is a 34-mer peptide which is the main component of amyloid deposits in FBD. As a first step toward shedding light for the discovery of amyloid inhibition drugs for FBD, we...

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Bibliographic Details
Published inJournal of industrial and engineering chemistry (Seoul, Korea) Vol. 17; no. 3; pp. 427 - 429
Main Authors Jeong, Keunhong, Chung, Woo Young, Kye, Young Sik, Kim, Dongwook
Format Journal Article
LanguageEnglish
Published Elsevier B.V 25.05.2011
한국공업화학회
Subjects
ABri
Aggregation rate
Alpha imager
Fluorescamine
화학공학
Fluorescamine
Alpha imager
Aggregation rate
ABri
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Summary:Familial British Dementia (FBD) is the early-onset autosomal dominant disorders characterized by cognitive impairment. The ABri is a 34-mer peptide which is the main component of amyloid deposits in FBD. As a first step toward shedding light for the discovery of amyloid inhibition drugs for FBD, we set the optimal conditions for quantification ABri peptide by the fluorescamine and alpha imager assay, which can provide a precise peptide quantity for determination of amyloid inhibition rate at micromolecular level and estimate aggregation rate by measuring monomer and dimer quantity which are filtered through 10,000 Da MW cutoff filter.
Bibliography:G704-000711.2011.17.3.022
http://www.cheric.org/article/897806
ISSN:1226-086X
1876-794X
DOI:10.1016/j.jiec.2010.09.017