Magnetic biosensor for the detection of Yersinia pestis

• Published in: Journal of microbiological methods Vol. 68; no. 2; pp. 218 - 224
• Main Authors: Meyer, Martin H.F., Stehr, Matthias, Bhuju, Sabin, Krause, Hans-Joachim, Hartmann, Markus, Miethe, Peter, Singh, Mahavir, Keusgen, Michael
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.02.2007; Elsevier Science
• Subjects: Animals; Antibodies, Bacterial - chemistry; Antigens, Bacterial - blood; AntiYPF1; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Cell Line; Fundamental and applied biological sciences. Psychology; Immunomagnetic Separation - instrumentation; Immunomagnetic Separation - methods; Magnetic beads; Magnetic detection; Mice; Microbiology; Plague - microbiology; Yersinia pestis; Yersinia pestis - isolation & purification; Magnetic detection; Magnetic beads; AntiYPF1; Yersinia pestis; AntiYPF 1; Biosensor; Bacteria; Method; Detection; Enterobacteriaceae
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2006.08.004

A novel type of magnetic-beads based magnetic biosensor is described for the detection of Yersinia pestis. Experiments were performed with the antigen fraction F1 of these bacteria. The magnetic sensor platform offers easy and reliable detection of Y. pestis by the use of magnetic beads for labelling and quantification in a single step due to their paramagnetic features. The system uses antiYPF1 antibodies as capture element on ABICAP® columns as core element of the magnetic sensor. Several immobilization methods for antibodies on polyethylene were exploited. The established biosensor has a linear detection range of 25–300 ng/ml Y. pestis antigen F1 and a detection limit of 2.5 ng/ml in buffer and human blood serum. The presented sensor system is small, simple, portable and therefore usable as off-lab detection unit for medical and warfare analytes.