Temperature and red cell metabolism: Effects of α-stat and pH-stat conditions

• Published in: Journal of thermal biology Vol. 15; no. 2; pp. 115 - 118
• Main Authors: Guppy, M., Sabaratnam, R., Whisson, M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1990; Elsevier
• Subjects: Biological and medical sciences; blood storage; Cell metabolism, cell oxidation; Cell physiology; erythrocyte; Fundamental and applied biological sciences. Psychology; glycolysis; histidine; lactate; Molecular and cellular biology; pentose phosphate pathway; Temperature; α-stat; Temperature; α-stat; glycolysis; pH; blood storage; lactate; pentose phosphate pathway; histidine; erythrocyte; Energy metabolism; Rat; Rodentia; Carbon dioxide; Red blood cell; Environmental factor; Lactates; Vertebrata; Blood cell; Mammalia; Histidine; Pentose phosphate pathway; Glycolysis; PH
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/0306-4565(90)90027-F

1. 1.Glycolytic and pentose phosphate pathway activities were measured in rat erythrocytes. The measurements were done at 35, 25 and 15°C under pH-stat and α-stat (constant ionization ratio of imidazole groups of histidine) regimes. 2. 2.The effects of temperature were less under the α-stat than under the pH-stat regime implying a significant role for histidine in the operation of the two major pathways of red cell metabolism. 3. 3.Current storage solutions show a Δ pH ° C which is intermediate between that of α-stat and that of pH-stat regimes. The implications of the data are that an α-stat storage regime woule lessen storage problems caused by decreased adenylates, ion imbalance and free radical production.