Synthesis, characterization and potential biomedical applications of N-succinimidyl ester functionalized, polypyrrole-coated polystyrene latex particles

• Published in: Colloid and polymer science Vol. 282; no. 12; pp. 1301 - 1307
• Main Authors: BOUSALEM, Smain, MANGENEY, Claire, CHEHIMI, Mohamed M, BASINSKA, Teresa, MIKSA, Beata, SLOMKOWSKI, Stanislaw
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.10.2004; Springer Nature B.V
• Subjects: Applied sciences; Atoms & subatomic particles; Copolymerization; Emulsion polymerization; Esters; Exact sciences and technology; Human; Latex; Morphology; Organic polymers; Physicochemistry of polymers; Polymers with particular properties; Polystyrene resins; Preparation, kinetics, thermodynamics, mechanism and catalysts; Pyrroles; Serum albumin; Spectrum analysis; Surface functionalization; Pyrrole derivative copolymer; Immobilization; Serum albumin; Latex particles; Experimental study; Polystyrene; Functional polymer; Protein; Organic adsorbent; Quantitative surface analysis; Latex; Submicron particle; Conducting polymers; Pyrrole copolymer; Electrophoretic mobility; Preparation; Covalent bond; Coated particle; Monodispersed particle; Styrene polymer; Oxidative polymerization; Polypyrrole
• ISSN: 0303-402X; 1435-1536
• DOI: 10.1007/s00396-004-1065-8

N-Succinimidyl ester functionalized polypyrrole-coated polystyrene latex particles (PS^sub E^-PPyNSE) were prepared by the in situ copolymerization of pyrrole and the active ester-functionalized pyrrole (pyrrole-NSE) in the presence of polystyrene latex particles. Polystyrene microspheres were prepared by emulsion polymerization (PS^sub E^) leading to particles having a diameter of 450 nm. These PS^sub E^ particles were precoated with poly(N-vinylpyrrolidone) prior to the in situ copolymerization of pyrrole and pyrrole-NSE. The initial comonomer concentration fractions were 25/75, 50/50 and 75/25 for pyrrole and pyrrole-NSE, respectively. The PPy-coated PS^sub E^particles were characterized in terms of morphology, particle size, electrophoretic mobility and chemical composition. The study of morphology by means of scanning electron microscopy showed roughening of the underlying PS^sub E^ particles owing to the addition of PPyNSE, the overlayer thickness of which was estimated to be around 7 nm. Moreover, loading PPyNSE overlayers resulted in a shift of the electrophoretic mobility from -5.31 μm cm/V s to a very small but positive value (0.082-0.112 μm cm/V s). X-ray photoelectron spectroscopy and IR spectroscopy permitted the detection of pyrrole-NSE repeat units at the surface indicating that pyrrole and pyrrole-NSE did indeed copolymerize. The PS^sub E^-PPyNSE particles were further evaluated as bioadsorbents of human serum albumin used as a test protein. For this study, PS^sub E^-PPyNSE^sub 50^ particles, synthesized from a comonomer feed ratio of 50/50 in pyrrole/pyrrole-NSE, were used and were shown to attach efficiently human serum albumin macromolecules with a maximum amount of 0.2 mg m^sup -2^.[PUBLICATION ABSTRACT]