Rapid propagation of Cucurbita pepo L. by culture of meristem tips

A tissue culture technique has been developed for the rapid multiplication of pumpkin ( Cucurbita pepo L.) clones. Meristem-tips from seedlings of cultivar ‘Cinderella’ were grown initially on MS medium containing 2.56 mg l −1 Kinetin and 8 mg l −1 IAA, and then transferred to experimental media. Ma...

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Bibliographic Details
Published inScientia horticulturae Vol. 24; no. 2; pp. 107 - 114
Main Authors Pink, D.A.C., Walkey, D.G.A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.01.1984
Elsevier
Subjects
Agronomy. Soil science and plant productions
Artificial regeneration. Forest nurseries. Planting
Biological and medical sciences
Forestry
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Kucurbita pepo L
meristem tip culture
tissue culture
Vegetative propagation. Micropropagation
Vegetative propagation. Sowing and planting. Harvesting
meristem tip culture
Kucurbita pepo L
tissue culture
In vitro vegetative propagation
Dicotyledones
Angiospermae
Cucurbitaceae
Tissue culture
Spermatophyta
Cucurbita pepo
Apex
Meristem
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Summary:A tissue culture technique has been developed for the rapid multiplication of pumpkin ( Cucurbita pepo L.) clones. Meristem-tips from seedlings of cultivar ‘Cinderella’ were grown initially on MS medium containing 2.56 mg l −1 Kinetin and 8 mg l −1 IAA, and then transferred to experimental media. Maximum shoot proliferation occurred on MS medium containing 1 mg l −1 BA and no auxin. Cultures were rooted after 2–3 weeks on MS medium containing 8 mg l −1 IAA and no cytokinin.
ISSN:0304-4238
1879-1018
DOI:10.1016/0304-4238(84)90141-9