Determination of the concentration of higher alcohols with enzyme coupled flow-injection analysis in model systems

• Published in: Analytica chimica acta Vol. 322; no. 3; pp. 131 - 139
• Main Authors: Burfeind, J., Weigel, B., Kretzmer, G., Schügerl, K., Huwig, A, Giffhorn, F.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.04.1996; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Enzymatic methods; Flow injection; Fundamental and applied biological sciences. Psychology; Higher alcohols; Immobilization of enzymes and other molecules; Immobilization techniques; Methods. Procedures. Technologies; Enzymatic methods; Higher alcohols; Flow injection; Alcohol oxidase; Ethanol; Enzyme; Amperometry; Oxidoreductases; Immobilized enzyme; Quantitative analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/0003-2670(95)00610-9

The activities of native and immobilized alcohol oxidases (AOD) originating from Pichia pastoris, Hansenula sp., Candida boidinii and Peniophora gigantea were compared with regard to the oxidation of various higher alcohols. Except Candida boidinii, the immobilized enzymes were applied in a flow-injection analysis system and investigated as a function of the various operation variables. During the oxidation O 2 is consumed which concentration was measured with an amperometric detector. At the same time H 2O 2 is produced, the concentration of which is used for the oxidation of a redox indicator (ABTS) catalysed by peroxidase and measured with a photometer. The amperometric method is simpler and more economical, but less sensitive. The photometric method consumes the dissolved redox indicator and peroxidase, but is highly sensitive especially for higher alcohols. The concentrations of n-propanol, n-butanol and n-pentanol can be measured with satisfactory sensitivity with immobilized alcohol oxidases originating from Pichia pastoris and Peniophera gigantea in a one molar potassium phosphate buffer (pH 7.5). The concentrations of isopropanol, octanol, dodecanol and benzylalcohol can be measured only when larger than 1 g l −1.