ECL resonance energy transfer-regulated “off-on” mode biosensor for the detection of miRNA-150-5p in triple negative breast cancer

• Published in: Biosensors & bioelectronics Vol. 240; p. 115663
• Main Authors: Zhong, Weiyao, Liang, Zihui, Zhao, He, Wang, Peilin, Li, Zhenrun, Shi, Jingwei, Ma, Qiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.11.2023
• Subjects: ECL-RET biosensor; Eu-doped MoS2 QDs; miRNA-150-5p detection; Triple negative breast cancer diagnosis; miRNA-150-5p detection; Eu-doped MoS2 QDs; ECL-RET biosensor; Triple negative breast cancer diagnosis
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2023.115663

MiRNAs played critical roles in triple negative breast cancer (TNBC) as potential biomarkers. Herein, an efficient signal “off-on” mode-biosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) was successfully constructed for the miRNA-150-5p determination in TNBC. The ECL-RET regulated-sensing platform consisted of NiMn-LDHs nanoflowers, the artificially assembled phospholipid bilayers and hairpin DNA-labeled Eu-doped MoS2 QDs. Firstly, Eu-doped MoS2 QDs with high quantum efficiency were prepared as the ECL-RET donors. And NiMn-layer double hydroxides (LDHs) nanoflowers with wide UV–vis absorption spectra as the ECL-RET acceptors. Secondly, due to the hairpin DNA structure, the closed distance between ECL-RET donor-acceptor pair can quench the luminescence signal of Eu-doped MoS2 QDs. When miRNA-150-5p was captured, the hairpin DNA structure changed to a rodlike configuration and enlarged the distance between Eu-doped MoS2 QDs and NiMn-LDHs. As a result, the recovery of ECL signal can be observed as a signal “turn off-on” mode. Furthermore, the hydrophilicity of the lipid bilayer can reduce the nonspecific adsorption and improve the flexibility of the hairpin DNA efficiently. Therefore, based on the ECL-RET regulation strategy, the biosensor was employed to detect miRNA-150-5p from 10 fM to 1 nM with a detection limit of 1.5 fM. The constructed biosensor can effectively differentiate TNBC patient tumor and healthy breast fibroadenoma. The ECL-RET regulation strategy provided a new biosensing pathway for ultrasensitive detection of biomolecules and promoted the development of diagnosis and treatment of TNBC.