Comparative approach for detection of biosurfactant-producing bacteria isolated from Ahvaz petroleum excavation areas in south of Iran

The current study was undertaken to compare four analytical methods including drop collapse, oil spreading, surface tension (SFT) measurements, and blood agar lysis tests for detection of biosurfactant-producing bacteria. Among 32 biosurfactant-producing bacteria isolated from Ahvaz oil fields, in s...

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Bibliographic Details
Published inAnnals of microbiology Vol. 58; no. 3; pp. 555 - 559
Main Authors Afshar, Shima, Lotfabad, Tayebe Bagheri, Roostaazad, Reza, Najafabadi, Abdolhossein Rouholamini, Noghabi, Kambiz Akbari
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.01.2008
University of Milan
Subjects
Applied Microbiology
Biological and medical sciences
Biomedical and Life Sciences
Fundamental and applied biological sciences. Psychology
Life Sciences
Medical Microbiology
Methods
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mycology
drop collapse
blood lysis
oil spreading
surface tension
biosurfactant
Biosurfactant
Lysis
Bacteria
Detection
Comparative study
Petroleum
Blood
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Summary:The current study was undertaken to compare four analytical methods including drop collapse, oil spreading, surface tension (SFT) measurements, and blood agar lysis tests for detection of biosurfactant-producing bacteria. Among 32 biosurfactant-producing bacteria isolated from Ahvaz oil fields, in south of Iran, 16 isolates (50%) exhibited highest biosurfactant production. Eleven isolates (MASH.1 to MASH.11) demonstrated a reduction in surface tension from 65 mN/m to less than 41 mN/m. The results showed that about 91% of these highly biosurfactant producers had the same response levels of “+++” and “+++” in the case of both SFT and oil spreading methods. Among these, seven isolates had the haemolysis diameter less than 1 cm or between 1 and 2 cm on blood agar. As 64% of the best biosurfactant producers did not completely lyses blood, the ability of biosurfactant-producers for haemolysis may not always be trustworthy. According to our data, there is a good consistency between oil spreading technique and surface tension. As a conclusion, oil spreading method is the fastest, simplest and most consistent analytical method to be suggested for accurate measurements of biosurfactant producers.
ISSN:1590-4261
1869-2044
DOI:10.1007/BF03175557