Use of multiplex PCR for diagnosis of bacterial infection respiratory mixed

Atypical bacteria grow very slowly in culture or they do not grow at all leading to delays in detection and diagnosis. PCR multiplex was performed on template DNAs extracted from seventy three collected specimens. Thirty seven showed positive indication for the presence of bacterial infection. The i...

Full description

Saved in:
Bibliographic Details
Published inMalaysian Journal of Microbiology Vol. 6; no. 1; pp. 1 - 6
Main Authors Al-ssum, R. M., Al-Malki, M. A. A.
Format Journal Article
LanguageEnglish
Published Malaysian Society for Microbiology 01.06.2010
Subjects
Chlamydia pneumonia
Epidemiology
Legionella pneumophila
Mycoplasma pneumoniae
Online AccessGet full text

Cover

More Information
Summary:Atypical bacteria grow very slowly in culture or they do not grow at all leading to delays in detection and diagnosis. PCR multiplex was performed on template DNAs extracted from seventy three collected specimens. Thirty seven showed positive indication for the presence of bacterial infection. The incidence of Mycoplasma pneumoniae, Chlamydia pneumonia and Legionella pneumophila as a single infecting agent was 31.5%, 27.5% and 20 % respectively. Dual agent infection caused by Mycoplasma + Chlamydia, Mycoplasma + Legionella and Legionella + Chlamydia was 24%, 20% and 15% respectively. Triple agent infection caused by Legionella + Mycoplasma + Chlamydia was 17.5%. The etiology of the infection was M. pneumoniae, L. pneumophila or C. pneumoniae as a single etiology or in combination of two or three organisms.
ISSN:2231-7538
1823-8262
2231-7538
DOI:10.21161/mjm.16309