Melittin–Lipid interaction: A comparative study using liposomes, micelles and bilayerdisks

Comparison of melittin interaction with liposomes, bilayer disks and micelles showed that melittin binding to lipid aggregates is largely dictated by the amount of highly curved areas in the aggregates. The PEG-stabilised bilayer disks were characterised by a combination of small angle neutron scatt...

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Bibliographic Details
Published inBiochimica et biophysica acta. Biomembranes Vol. 1778; no. 10; pp. 2210 - 2216
Main Authors Lundquist, Anna, Wessman, Per, Rennie, Adrian R., Edwards, Katarina
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.10.2008
Subjects
Bilayer disk
Liposome
Melittin
Micelle
Neutron scattering
PEG–lipid
Micelle
Liposome
Bilayer disk
Melittin
Neutron scattering
PEG–lipid
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Summary:Comparison of melittin interaction with liposomes, bilayer disks and micelles showed that melittin binding to lipid aggregates is largely dictated by the amount of highly curved areas in the aggregates. The PEG-stabilised bilayer disks were characterised by a combination of small angle neutron scattering, cryo-transmission electron microscopy and dynamic light scattering. Importantly, the theoretically foreseen partial segregation of the lipid components, important for maintaining the structure of the bilayer disk, was confirmed. Steady state fluorescence spectroscopy indicated that melittin mainly resides at the rim of the bilayer disks. Results of the present study help increase the understanding of the mechanisms behind, and the physico-chemical factors affecting, melittin–lipid interaction. We suggest that bilayer disks, due to their stable structure, constitute interesting vehicles for transport of peptides that have high propensity to associate with lipid surfaces of high curvature.
ISSN:0005-2736
1879-2642
DOI:10.1016/j.bbamem.2008.05.009