Effect of 17β-estradiol on the immunocompetence of Japanese sea bass (Lateolabrax japonicus)

• Published in: Environmental toxicology and chemistry Vol. 28; no. 8; pp. 1722 - 1731
• Main Authors: Thilagam, Harikrishnan, Gopalakrishnan, Singaram, Bo, Jun, Wang, Ke-Jian
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Periodicals, Inc 01.08.2009
• Subjects: 17β-Estradiol; endocrine-disrupting chemicals; estradiol; Estrogen; Immune parameters; immunocompetence; Immunotoxicity; Lateolabrax japonicus; long term effects; marine fish; water pollution
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1897/08-642.1

Environmental contaminants can interfere with hormonal regulation in both vertebrates and invertebrates, and these contaminants may disrupt the endocrine system of human and other organisms. Evidence is growing that contaminants may be partly responsible for the observed increase of disease in marine organisms by adversely affecting their immunity. Fish are commonly used as sentinel organisms in vertebrate immunotoxicology; however, to date, studies have been undertaken only on a single size group of fish (juvenile/adult) and for acute exposure. In the present study, Lateolabrax japonicus fingerlings and juveniles were exposed to two sublethal concentrations (200 and 2,000 ng/L) of 17β-estradiol (E2) for 30 d under laboratory conditions, and alterations in immune parameters comprising differential leukocyte count, respiratory burst, myeloperoxidase, immunoglobulin levels, serum lysozyme, and bactericidal activity were investigated to establish whether estrogen produced immunomodulation and to understand the effects of long-term exposure on these immune parameters in fish fingerlings and juveniles. The results revealed a significant elevation of respiratory burst activity, myeloperoxidase, immunoglobulin levels, and differential leukocyte counts of the fish exposed to estrogen compared to the control. The remaining parameters were significantly reduced in the experimental groups when compared to the control. The results indicated that sublethal E2 exposure induced immunomodulation in both fingerling and juvenile L. japonicus, and the changes caused by estrogen might affect the function of immune system in fish.