ANALYSIS OF THE DNAS OF EPSTEIN-BARR VIRUS (EBV) DERIVED FROM TWO EBV GENOME-POSITIVE EPITHELIAL/HYBRID CELL LINES (NPC-KT AND A2L/AH)

• Published in: Nippon Jibi Inkoka Gakkai Kaiho Vol. 90; no. 8; pp. 1177 - 1180
• Main Authors: TAKIMOTO, TORU, MIYAZAKI, TAMEO, IWAWAKI, JUNICHI, ISHIKAWA, SHIGERU, TANAKA, SAICHIROH, MASUDA, KOH, YOSHIZAKI, TOMOKAZU
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Oto-Rhino-Laryngological Society of Japan, Inc 1987
• Subjects: A2L/AH; Carcinoma; Cell Line, Transformed; DNA; DNA, Viral - analysis; Epithelial Cells; Epstein-Barr virus; Genes, Viral; Herpesvirus 4, Human - genetics; Humans; Hybrid Cells - analysis; Molecular Weight; Nasopharyngeal Neoplasms; NPC-KT
• ISSN: 0030-6622; 1883-0854
• DOI: 10.3950/jibiinkoka.90.1177

The authors recently reported the establishment of two Epstein-Barr virus (EBV)-producing epithelial/hybrid cell lines (A2L/AH and NPC-KT), A2L/AH cells were derived from the fusion of epithelial cell line (Ad-AH) with non-producer EBV genome-positive lymphoblastoid cells transformed with the B95-8 strain of EBV. NPC-KT cells were derived from the fusion of Ad-AH cells with primary nasopharyngeal carcinoma cells. In this study, viral DNAs derived from A2L/AH and NPC-KT cells were analyzed in comparison with viral DNA derived from B95-8 cells by use of the restriction endonuclease EcoRI and Hind III and separation of fragments in 0.4% agarose. The digestion fragments of these two viral DNAs were obviously similar, but could be distiguished each other. The molecular weight of DNA of virus from NPC-KT cells was approximately 181K base pairs (Kb), which was 12Kb larger than that of B95-8 virus DNA. Key words: NPC-KT, A2L/AH, Epstein-Barr virus, DNA A90-1177-51360