Markhasphingolipid A, new phytosphingolipid from the leaves of Markhamia stipulata var. canaense V.S. Dang

• Published in: Natural product research Vol. 34; no. 13; pp. 1820 - 1826
• Main Authors: Ngo, Trong Nghia, Nguyen, Nu Dan Phuong, Nguyen, Ngoc Thien Ly, Pham, Nguyen Kim Tuyen, Phan, Nhat Minh, Bui, Trong Dat, Dang, Van Son, Tran, Cong Luan, Mai, Dinh Tri, Nguyen, Tan Phat
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 02.07.2020; Taylor & Francis Ltd
• Subjects: Bignoniaceae; Biotechnology; Cancer; Chromatography; cytotoxic activity; Cytotoxicity; Glycerol; Markhamia stipulata; Markhasphingolipid A; n-Hexane; NMR; Nuclear magnetic resonance; Toxicity; Tumor cell lines
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2018.1561686

From the leaves of Markhamia stipulata var. canaense V.S. Dang, one new phytosphingolipid, named markhasphingolipid A (6) together with five known compounds, 4',7-O-dimethylapigenin (1), narigenin (2), tectoquinone (3), mollic acid (4), 1-hexadecanoyl-sn-glycerol (5) were classified by various chromatographic methods. Their structures were designated by IR, UV, HR-ESI-MS, HR-ESI-MS/MS and NMR experiments. All compounds were recognized for the first time from this species. The cytotoxicity of all n-hexane fractions and isolated compounds (5 & 6) against three human cancer cell lines (HeLa, HepG2, and MCF-7) were evaluated by SRB assay. All n-hexane fractions expressed cytotoxic effect on three tested cancer cell lines (at the concentration of 100 μg/mL, percent of cytotoxicity ranged from 55.81% to 95.83%) as well as compound 5 (IC 50 ranged from 48.51 to 63.30 μM) whereas fraction H.I and compound 6 did not show activity.