Crystallization and preliminary X-ray investigation of recombinant human interleukin 10

Crystals of recombinant human interleukin 10 have been grown from solutions of ammonium sulfate. The crystals are tetragonal, space group P4(1)2(1)2 or P4(3)2(1)2; the unit cell axes are a = 36.5 A and c = 221.9 A. There is the equivalent of one polypeptide chain in the asymmetric unit. The crystals...

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Bibliographic Details
Published inProteins, structure, function, and bioinformatics Vol. 22; no. 2; p. 187
Main Authors Cook, W J, Windsor, W T, Murgolo, N J, Tindall, S H, Nagabhushan, T L, Walter, M R
Format Journal Article
LanguageEnglish
Published United States 01.06.1995
Subjects
Ammonium Sulfate - chemistry
Animals
CHO Cells - metabolism
Cricetinae
Escherichia coli - metabolism
Humans
Interleukin-10 - chemistry
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
X-Ray Diffraction
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Summary:Crystals of recombinant human interleukin 10 have been grown from solutions of ammonium sulfate. The crystals are tetragonal, space group P4(1)2(1)2 or P4(3)2(1)2; the unit cell axes are a = 36.5 A and c = 221.9 A. There is the equivalent of one polypeptide chain in the asymmetric unit. The crystals are stable to X-rays and diffract to at least 2.5 A resolution.
ISSN:0887-3585
DOI:10.1002/prot.340220211