A CRISPR/Cas9-Based Study of CgloRPCYG, a Gene That Regulates Pathogenicity, Conidial Yield, and Germination in Colletotrichum gloeosporioides

The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and co...

Full description

Saved in:
Bibliographic Details
Published inAgronomy (Basel) Vol. 13; no. 7; p. 1681
Main Authors Zhang, He, Xia, Yu-Qi, Xia, Yang, Zhang, Meng-Ting, Ye, Zi, Sun, Rui-Qing, Liu, Xiao-Mei, Pu, Jin-Ji
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.07.2023
Subjects
Agricultural production
Amino acids
Anthracnose
Colletotrichum gloeosporioides
complementary strain
CRISPR
CRISPR/Cas9 knockout mutants
E coli
Editing
filamentous fungus
Fruits
Fungi
Gene silencing
Genes
Genetic engineering
Genomes
Genomics
Germination
Mutants
Pathogenicity
Pathogens
Phenotypes
Phylogenetics
Plant diseases
Plant protection
Plasmids
Polyethylene glycol
United States > US
China
Online AccessGet full text

Cover

More Information
Summary:The filamentous fungus Colletotrichum gloeosporioides is the causative agent of one of the most serious diseases that damage plant fruit. In this study, we discovered and experimentally characterized a new gene in Colletotrichum gloeosporioides named CgloRPCYG. The CRISPR/Cas9 knockout mutant and complementary strain of CgloRPCYG were then obtained by polyethylene glycol (PEG)-mediated protoplast transformation to determine the related phenotypes. Compared with the wild-type strain and complementary mutant, the pathogenicity of the CRISPR/Cas9 knockout mutant was significantly decreased, the conidial yield was significantly reduced, and conidial germination was significantly delayed. These data indicate that CgloRPCYG contributes to pathogenicity, conidial yield, and germination in C. gloeosporioides. The successful application of the CRISPR/Cas9 system in C. gloeosporioides also confirms its utility in filamentous fungi for fundamental research and practical application. Furthermore, CgloRPCYG is a potential target gene for use in the development of plant protection technologies, such as spray-induced gene silencing, with the aim of controlling plant anthracnose disease caused by C. gloeosporioides.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy13071681