Influence of Cyclophosphamide on the Delayed Hypersensitivity in the Mouse After Immunization with Histocompatibility Antigen

• Published in: The Journal of immunology (1950) Vol. 113; no. 6; pp. 1801 - 1806
• Main Authors: Kerckhaert, Joseph A, van den Berg, Gert J, Hofhuis, Frans M. A
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.12.1974
• Subjects: Animals; Biological Assay; Cyclophosphamide - pharmacology; Female; Graft vs Host Reaction; Hindlimb; Histocompatibility Antigens; Hypersensitivity, Delayed - immunology; Immunization; Lymphocytes - immunology; Mice; Mice, Inbred BALB C; Mitomycins - pharmacology; Spleen - cytology
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.113.6.1801

Abstract The use of delayed hypersensitivity (DH) reactions as a measure of the immunity against histocompatibility antigens in mice has been described. The DH was only provoked to a measurable level if the sensitization was preceded by cyclophosphamide (CY) treatment. CY treatment before i.p. immunization caused an initial suppression of the DH. This was followed by an enhancement when the interval between immunization and challenge was 10 days. Immunization i.p. with allogeneic or F1 spleen cells only resulted in a sensitization for DH if preceded by CY treatment. Differences in dose response curves between i.p. administered allogeneic and semi-allogeneic cells after CY treatment suggested that the graft-vs-host reaction (GvH) interfered with the DH. Comparison of 1) DH reaction after i.p. immunization and CY treatment and 2) GvH activity of cells from different lymphoid organs gave further indications of this interference.