Analysis of splice donor and acceptor site function in a transposable gene trap derived from the maize element Activator

• Published in: Molecular & general genetics Vol. 249; no. 1; p. 91
• Main Authors: Nussaume, L, Harrison, K, Klimyuk, V, Martienssen, R, Sundaresan, V, Jones, J.D.G. (John Innes Inst., Norwich (United Kingdom). Sainsbury Lab.)
• Format: Journal Article
• Language: English
• Published: Germany 01.01.1995
• Subjects: Arabidopsis - genetics; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA Primers; DNA Transposable Elements; DNA, Complementary - biosynthesis; EXPRESION GENICA; EXPRESSION DES GENES; GENE; GENES; Genes, Plant; Glucuronidase - biosynthesis; Introns; Models, Genetic; Molecular Sequence Data; Nicotiana - genetics; Plants, Toxic; Polymerase Chain Reaction; Recombinant Proteins - biosynthesis; RNA Splicing; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RNA, Plant - analysis; RNA, Plant - biosynthesis; Species Specificity; Transcription, Genetic; Transfection; VECTEUR GENETIQUE; VECTORES GENETICOS; ZEA MAYS; Zea mays - genetics
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF00290240

Gene trap vectors have been used in insertional mutagenesis in animal systems to clone genes with interesting patterns of expression. These vectors are designed to allow the expression of a reporter gene when the vector inserts into a transcribed region. In this paper alternative splicing events were examine that result in the expression of a GUS reporter gene carried on a Ds element which has been designed as a gene trap vector for plants. A rapid and reliable method was developed based on PCR to study such events. Many splice donor sites were observed in the 3' Ac border. The relative frequency of utilisation of certain splice donor and acceptor sites differed between tobacco and Arabidopsis. A higher stringency of splicing was observed in Arabidopsis.