The N- and C-terminal boundaries of myelin basic protein determinants required for encephalitogenic and proliferative responses of Lewis rat T cells

• Published in: Journal of neuroimmunology Vol. 26; no. 3; pp. 201 - 211
• Main Authors: Mannie, M.D., Paterson, P.Y., U'Prichard, D.C., Flouret, G.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.03.1990; Amsterdam Elsevier; New York, NY
• Subjects: Adoptive transfer; Animals; Biological and medical sciences; Cell Division; Cell Line; Encephalitis - immunology; Encephalitis - pathology; Encephalitogenic determinant; Epitopes; Experimental autoimmune (allergic) encephalomyelitis; Immunization, Passive; Immunochemistry; Male; Medical sciences; Myelin basic protein; Myelin Basic Protein - immunology; Nervous system involvement in other diseases. Miscellaneous; Neurology; Peptides - chemical synthesis; Peptides - immunology; Proliferation, in vitro; Rats; Rats, Inbred Lew; T lymphocyte; T-Lymphocytes - pathology; T-Lymphocytes - physiology; Encephalitogenic determinant; T lymphocyte; Experimental autoimmune (allergic) encephalomyelitis; Proliferation, in vitro; Myelin basic protein; Adoptive transfer; Cell proliferation; Allergy; Nervous system diseases; N terminal»-Sequence; Encephalomyelitis; Rat; Myelin; Rodentia; Autoimmune disease; In vitro; C terminal-Sequence; Vertebrata; Experimental disease; Mammalia; Synthetic product; Basic protein; Encephalitogenic protein; T»-Lymphocyte; Aminoacid sequence
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(90)90002-5

Highly purified synthetic peptides representing portions of the 68–86 sequence of guinea pig (GP) myelin basic protein (GPMBP) were used to define the N- and C-termini of encephalitogenic determinants that cause experimental autimmune encephalomyelitis (EAE) in Lewis rats. Each peptide was tested for: (a) induction of EAE, (b) in vitro potentiation of EAE transfer activity by GPMBP-sensitized lymph node cells (LNC), (c) in vitro proliferation of GPMBP-sensitized LNC, and (d) in vitro proliferation of a GPMBP-reactive line of EAE-inducing T cells. In these bioassays, the general rank order of potency was: GPMBP≥GP68−86≥GP72–86>[G 84]GP68−86≥GP68−84⪢GP75−85≥GP75−84 = virtually no activity. These results demonstrate that the encephalitogenic region is bounded by the 72–74 and 84–86 sequences. Further evidence presented herein indicates that the 75–84 sequence contains the primary antigenic features for specific T cell recognition of the encephalitogenic region.