The 34 kilodalton insulin-like growth factor binding proteins in human cerebrospinal fluid and the A673 rhabdomyosarcoma cell line are human homologues of the rat BRL-3A binding protein

• Published in: Biochemical and biophysical research communications Vol. 163; no. 2; pp. 875 - 881
• Main Authors: Romanus, Joyce A., Tseng, Lucy Y.-H., Yang, Yvonne W.-H., Rechler, Matthew M.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.09.1989; Elsevier
• Subjects: Amniotic Fluid - metabolism; Analytical, structural and metabolic biochemistry; Animals; Binding and carrier proteins; Biological and medical sciences; Blotting, Northern; Blotting, Western; Carrier Proteins - metabolism; Cell Line; DNA - genetics; DNA Probes; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Humans; Insulin-Like Growth Factor Binding Proteins; Molecular Weight; Nucleic Acid Hybridization; Proteins; Rats; Rhabdomyosarcoma - metabolism; Sequence Homology, Nucleic Acid; Somatomedins - cerebrospinal fluid; Tumor Cells, Cultured - metabolism; SDS; BP-25; CSF; kb; IGF; kDa; BP-3A; BP-53; bp; Human; Cell culture; Molecular hybridization; Rat; Molecular structure; Nucleotide sequence; Rodentia; Homology; Cerebrospinal fluid; Binding protein; Vertebrata; Mammalia; Cell line; Complementary DNA; Immunoblotting assay; Serum
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(89)92303-6

Three members of a family of insulin-like growth factor binding proteins have been identified by nucleotide sequencing of cDNA clones: the binding subunit of the 150 kDa IGF-binding protein complex in human serum, the 30 kDa IGF binding protein in human amniotic fluid, and a 30 kDa binding protein (BP-3A) isolated from the rat BRL-3A cell line. The present study demonstrates by molecular hybridization and immunoreactivity that the human counterpart of rat BP-3A is a 34 kDa IGF binding protein that is present in human cerebrospinal fluid and is synthesized and secreted by the A673 human rhabdomyosarcoma cell line.