Expression of CGRP mRNAs in the pink salmon, Oncorhynchus gorbuscha

• Published in: Peptides (New York, N.Y. : 1980) Vol. 14; no. 5; pp. 977 - 981
• Main Authors: Maubras, L., Taboulet, J., Pidoux, E., Lasmoles, F., Julienne, A., Milhaud, G., Benson, A.A., Moukhtar, M.S., Cressent, M.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.09.1993; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Blotting, Northern; Brackish; Calcitonin Gene-Related Peptide - genetics; CGRP RNAs; Female; Freshwater; Fundamental and applied biological sciences. Psychology; Gene expression; Marine; Molecular and cellular biology; Molecular genetics; Oncorhynchus - metabolism; Oncorhynchus gorbuscha; Organ Specificity - physiology; Plasma CGRP; Radioimmunoassay; Reproduction - physiology; Reproductive cycle; RNA, Messenger - biosynthesis; Salmon; Plasma CGRP; CGRP RNAs; Salmon; Reproductive cycle; Vertebrata; Messenger RNA; Pisces; Oncorhynchus gorbuscha; Gene expression; Calcitonine gene related peptide; Neuropeptide
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/0196-9781(93)90074-Q

We report the isolation of calcitonin gene-related peptide (CGRP) mRNAs and expression of these RNAs in different tissues in the pink salmon, Oncorhynchus gorbuscha. Hybridization of poly(A +) RNAs indicated a mature CGRP RNA of 1.1 kb. The CGRP-like immunoreactivity occurring in tissues and plasma had the same relative molecular weight as the synthetic molecule. Variations in CGRP plasma levels were observed during migration, spawning, and postspawning states. These data suggest that CGRP may play an important role during the reproductive cycle of salmon.