DNA nanocrane-based catalysts for region-specific protein modification

This paper describes the development and performance of catalytic DNA-based nanocranes for the controlled modification of wild-type proteins. We show that the position of the catalyst offers control over the region of modification, and that reversible interactions between the catalytic structure and...

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Bibliographic Details
Published inOrganic & biomolecular chemistry Vol. 22; no. 7; pp. 1447 - 1452
Main Authors Keijzer, Jordi F, Albada, Bauke
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 14.02.2024
Subjects
Catalysis
Catalysts
Deoxyribonucleic acid
Deoxyribozymes
DNA
DNA - chemistry
DNA, Catalytic - metabolism
Protein Processing, Post-Translational
Proteins
Proteins - chemistry
Thrombin
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Summary:This paper describes the development and performance of catalytic DNA-based nanocranes for the controlled modification of wild-type proteins. We show that the position of the catalyst offers control over the region of modification, and that reversible interactions between the catalytic structure and thrombin enable trigger-responsive modification, even in cell lysate. Catalytic DNA-based nanocranes are developed for modification of a target protein in E. coli cell lysate. Trigger-responsive protein-selective modification in this demanding environment was also shown, revealing the potential of such constructs.
Bibliography:Electronic supplementary information (ESI) available: Synthetic procedures and analytical data, analytical data, details of protein modification experiments. A supplementary video associated to Fig. 3E. See DOI
https://doi.org/10.1039/d3ob02025h
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ISSN:1477-0520
1477-0539
DOI:10.1039/d3ob02025h