Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

ABSTRACT Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA)...

Full description

Saved in:
Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 86; no. 10; pp. 1459 - 1461
Main Authors Kawai, Natsuki, Hirakawa, Yuki, Matsumoto, Shinya, Itai, Akihiro, Matsunaga, Kayoko, Narita, Hiroshi, Momma, Keiko
Format Journal Article
LanguageEnglish
Published 23.09.2022
Online AccessGet full text

Cover

More Information
Summary:ABSTRACT Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbac123