Modification of rat caecal microbial biotransformation activities by dietary saccharin

Male Sprague-Dawley rats were fed a purified fibre-free diet containing 5% (w/w) sodium saccharin for 4 weeks or 20 weeks and changes in caecal bacterial numbers and enzyme activities (endogenous ammonia production, beta-glucosidase, beta-glucuronidase, nitrate reductase, nitroreductase, aryl sulpha...

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Bibliographic Details
Published inToxicology (Amsterdam) Vol. 36; no. 2-3; p. 253
Main Authors Mallett, A K, Rowland, I R, Bearne, C A
Format Journal Article
LanguageEnglish
Published Ireland 01.08.1985
Subjects
Ammonia - metabolism
Animals
Bacteria - drug effects
Bacteria - metabolism
Biotransformation
Cecum - drug effects
Cecum - microbiology
Glucuronidase - analysis
Male
Nitrate Reductase
Nitrate Reductases - analysis
Nitroreductases
Oxidoreductases - analysis
Rats
Rats, Inbred Strains
Saccharin - toxicity
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Summary:Male Sprague-Dawley rats were fed a purified fibre-free diet containing 5% (w/w) sodium saccharin for 4 weeks or 20 weeks and changes in caecal bacterial numbers and enzyme activities (endogenous ammonia production, beta-glucosidase, beta-glucuronidase, nitrate reductase, nitroreductase, aryl sulphatase) determined in vitro. Saccharin treatment gave marked caecal enlargement but had no effect on bacterial concentration at either treatment period, and significantly decreased beta-glucuronidase, nitrate reductase and sulphatase activities/g caecal contents. The incubation of a suspension of caecal contents from control rats with saccharin (75 mM) in vitro inhibited beta-glucuronidase and nitrate reductase activities, and ammonia production from endogenous substrates. Such changes may decrease the rate of formation of toxic bacterial products in the hindgut.
ISSN:0300-483X
1879-3185
DOI:10.1016/0300-483X(85)90058-7