Isolation and spectral characteristics of four toxins from the dinoflagellate Ptychodiscus brevis

Four ichthyotoxins were isolated from crude toxic extracts of Ptychodiscus brevis using a combination of solvent partitioning, thin layer chromatography and reversed phase high pressure liquid chromatography. The toxins were analyzed by mass, infrared, 1H- and 13C-NMR spectroscopy and were found to...

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Bibliographic Details
Published inToxicon (Oxford) Vol. 24; no. 11-12; p. 1075
Main Authors Whitefleet-Smith, J, Boyer, G L, Schnoes, H K
Format Journal Article
LanguageEnglish
Published England 1986
Subjects
Animals
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Dinoflagellida - analysis
Magnetic Resonance Spectroscopy
Marine Toxins - isolation & purification
Marine Toxins - toxicity
Mass Spectrometry
Poecilia
Spectrophotometry, Infrared
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Summary:Four ichthyotoxins were isolated from crude toxic extracts of Ptychodiscus brevis using a combination of solvent partitioning, thin layer chromatography and reversed phase high pressure liquid chromatography. The toxins were analyzed by mass, infrared, 1H- and 13C-NMR spectroscopy and were found to constitute two structural families of two toxins each: brevetoxins 1 and 2 and brevetoxins 3 and 4. Comparison with literature data indicates that brevetoxins 1 and 2 are identical to the previously described and characterized 11-ring polyether toxins brevetoxins C and B, respectively. The other two compounds (brevetoxins 3 and 4) also represent a structural pair (with chloroacetone and alpha-methylene-propanal side chains, respectively) which has a different, but related, basic ring-structure.
ISSN:0041-0101
DOI:10.1016/0041-0101(86)90134-0