Identification and characterization of the nolYZ genes of Bradyrhizobium japonicum

• Published in: Molecular plant-microbe interactions Vol. 7; no. 2; pp. 173 - 180
• Main Authors: Dockendorff, T.C, Sharma, A.J, Stacey, G
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.03.1994
• Subjects: ADN; Agronomy. Soil science and plant productions; Amino Acid Sequence; AMINO ACID SEQUENCES; BACTERIA; Bacterial Proteins - genetics; Base Sequence; Binding Sites; Biological and medical sciences; BRADYRHIZOBIUM; DELETIONS; DNA; DNA BINDING PROTEINS; DNA, Bacterial; Economic plant physiology; EXPRESION GENICA; EXPRESSION DES GENES; FORMATION DE NODOSITES; Fundamental and applied biological sciences. Psychology; GENE; GENE EXPRESSION; GENES; Genes, Bacterial; GENETIC REGULATION; GENETICA; GENETICS; GENETIQUE; GLYCINE MAX; INDUCED MUTATION; Isoflavones - pharmacology; MACROPTILIUM ATROPURPUREUM; MOLECULAR SEQUENCE DATA; MUTACION INDUCIDA; Mutagenesis, Insertional; MUTATION PROVOQUEE; NODULACION; NODULATION; NOLY GENE; NOLZ GENE; NUCLEOTIDE SEQUENCE; OPEN READING FRAMES; PROTEINAS; PROTEINE; PROTEINS; Rhizobiaceae - genetics; ROOT NODULATION; SECUENCIA NUCLEICA; Sequence Deletion; SEQUENCE NUCLEIQUE; STRUCTURAL GENES; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiosis - genetics; VIGNA RADIATA; VIGNA UNGUICULATA; Symbiosis; Macroptilium atropurpureum; Nucleotide sequence; Bradyrhizobium japonicum; Vigna radiata; Vigna unguiculata; Nodulation; Vegetal microorganism relation; Gene organization; Gene expression; Glycine max; Isoflavone derivatives; Grain legume; Regulation(control); Leguminosae; Dicotyledones; Angiospermae; Bacteria; Spermatophyta; Rhizobiaceae; Aminoacid sequence; Structural analysis
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI-7-0173

Characterization of an isoflavone-inducible locus closely linked to the common nod genes of Bradyrhizobium japonicum USDA110 led to the discovery of two open reading frames, designated nolY and nolZ. These open reading frames are preceded by a sequence with strong similarity to a consensus NodD-binding site (nod box). Studies utilizing a nolZ'-'lacZ fusion indicated that inducible expression is dependent upon both NodD1 and NodW, transcriptional regulators that are required for the expression of the common nodulation genes (e.g., nodYABC) of B. japonicum. A deletion mutation within nolY produced only slight defects in nodulation of soybeans, siratro, and cowpeas, but stronger defects were observed in nodulation of mung beans. An insertion mutation within nolZ showed no nodulation defects in the host plants tested. Competition assays for nodule occupancy in soybeans did not show any decrease in the competitiveness of a nolY mutant, nor did a nolY mutant show any detectable alteration in the production of lipooligosaccharide nodulation signals.