Secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles

• Published in: Molecular plant-microbe interactions Vol. 9; no. 1; pp. 39 - 46
• Main Authors: Boer, J.M. de (Wageningen Agricultural University, Wageningen, The Netherlands.), Smant, G, Goverse, A, Davis, E.L, Overmars, H.A, Pomp, H.R, Gent-Pelzer, M. van, Zilverentant, J.F, Stokkermans, J.P.W.G, Hussey, R.S
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.01.1996
• Subjects: Animals; Antibodies, Helminth - immunology; Antibodies, Monoclonal; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Antigens, Helminth - isolation & purification; Biochemistry. Physiology. Immunology. Molecular biology; Biological and medical sciences; Blotting, Western; CHIMIE; CITOPLASMA; Cross Reactions; CYTOPLASME; Cytoplasmic Granules - chemistry; Cytoplasmic Granules - ultrastructure; Digestive System - chemistry; Digestive System - ultrastructure; ESOFAGO; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; GLANDE ANIMALE; GLANDULAS ANIMALES; GLOBODERA ROSTOCHIENSIS; Helminth Proteins - immunology; Helminth Proteins - isolation & purification; Host-Parasite Interactions; IMMUNOFLUORESCENCE; IMMUNOLOGIE; INMUNOFLUORESCENCIA; INMUNOLOGIA; Invertebrates; LARVAS; LARVE; Microscopy, Immunoelectron; Nemathelminthia. Plathelmintha; Nematoda - chemistry; Nematoda - growth & development; Nematoda - pathogenicity; Nematoda - ultrastructure; NEMATODE DES PLANTES; NEMATODOS DE LAS PLANTAS; OESOPHAGE; Physiology. Development; PROTEINAS; PROTEINE; QUIMICA; REACCION ANTIGENO-ANTICUERPO; REACTION ANTIGENE ANTICORPS; Solanum tuberosum - parasitology; Species Specificity; Virulence; Host parasite relation; Digestive system; Solanum tuberosum; Abdominal gland; Globodera rostochiensis; Hybridoma; Monoclonal antibody; Method; Esophagus; Antigen; Pest; Dicotyledones; Angiospermae; Secretory protein; Cross reaction; Helmintha; Immunoblotting assay; Nemathelminthia; Spermatophyta; Tuber plant; Solanaceae; Secretion granule; Invertebrata; Nematoda
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI-9-0039

Sodium dodecyl sulfate-extracted proteins from second stage juveniles (J2) of the potato cyst nematode Globodera rostochiensis were fractionated by preparative continuous flow electrophoresis, and monoclonal antibodies (MAbs) were raised against the 38- to 40.5-kDa protein fraction. Screening of the hybridoma culture fluids by immunofluorescence microscopy of J2 resulted in the identification of 12 MAbs that bound specifically to the subventral esophageal glands. On Western blots of J2 these MAbs identified four protein bands with apparent molecular masses of 30, 31, 39, and 49 kDa. Immunoelectron microscopy with one of these MAbs showed an intense labeling of the electron dense core of the secretory granules in the subventral gland cells of J2. It is concluded that one or more of these proteins are localized within these secretory granules. Immunofluorescence microscopy of J2 from other plant parasitic nematode species showed that most of these MAbs also bind to the subventral glands of G. pallida and G. tabacum but not of Heterodera schachtii, H. glycines, Meloidogyne incognita, or M. hapla