Solid state nanopores for gene expression profiling

Recently, nanopore technology has been introduced for genome analysis. Here we show results related to the possibility of preparing “engineered solid state nanopores”. The nanopores were fabricated on a suspended Si 3N 4 membrane by Focused Ion Beam (FIB) drilling and chemically functionalized in or...

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Bibliographic Details
Published inSuperlattices and microstructures Vol. 46; no. 1; pp. 59 - 63
Main Authors Mussi, V., Fanzio, P., Repetto, L., Firpo, G., Valbusa, U., Scaruffi, P., Stigliani, S., Tonini, G.P.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.07.2009
Subjects
Biosensor
Functionalization
Gene expression
Ionic current
Nanopore
Nanopore
Ionic current
Functionalization
Gene expression
Biosensor
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Summary:Recently, nanopore technology has been introduced for genome analysis. Here we show results related to the possibility of preparing “engineered solid state nanopores”. The nanopores were fabricated on a suspended Si 3N 4 membrane by Focused Ion Beam (FIB) drilling and chemically functionalized in order to covalently bind oligonucleotides (probes) on their surface. Our data show the stable effect of DNA attachment on the ionic current measured through the nanopore, making it possible to conceive and develop a selective biosensor for gene expression profiling.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0749-6036
1096-3677
DOI:10.1016/j.spmi.2008.09.003