Increased Soluble Cytoplasmic Bcl-2 Protein Serum Levels and Expression and Decreased Fas Expression in Lymphocytes and Monocytes in Juvenile Dermatomyositis

To evaluate soluble Fas antigen (sFas), sFas ligand (sFasL), soluble tumor necrosis factor-related apoptosis-inducing ligand, and soluble cytoplasmic Bcl-2 protein (sBcl-2) serum levels, Fas and Bcl-2 expressions in T and B lymphocytes and monocytes and relations with erythrocyte sedimentation rate,...

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Published inJournal of rheumatology Vol. 45; no. 11; p. 1577
Main Authors Liphaus, Bernadete L, Sallum, Adriana E M, Aikawa, Nadia E, Kiss, Maria Helena B, Carrasco, Solange, Palmeira, Patricia, Lima, Laila, Silva, Clovis A, Goldenstein-Schainberg, Claudia, Carneiro-Sampaio, Magda
Format Journal Article
LanguageEnglish
Published Canada 01.11.2018
Subjects
MONOCYTES
APOPTOSIS
JUVENILE DERMATOMYOSITIS
FASL AND TRAIL
SOLUBLE BCL-2
LYMPHOCYTES
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Summary:To evaluate soluble Fas antigen (sFas), sFas ligand (sFasL), soluble tumor necrosis factor-related apoptosis-inducing ligand, and soluble cytoplasmic Bcl-2 protein (sBcl-2) serum levels, Fas and Bcl-2 expressions in T and B lymphocytes and monocytes and relations with erythrocyte sedimentation rate, C-reactive protein (CRP), Childhood Myositis Assessment Scale, and manual muscle testing in juvenile dermatomyositis (JDM). Serum levels were determined by ELISA and peripheral cell expressions by flow cytometry for patients with JDM or juvenile idiopathic arthritis (JIA), and healthy controls. Patients with JDM had increased sBcl-2, which correlated with CRP. Expression of Bcl-2 was increased and expression of Fas was decreased in CD3+, CD4+, and CD8+ T lymphocytes compared with JIA and/or healthy controls. Patients with JDM presented a unique apoptosis-related proteins profile, which may contribute to disease development.
ISSN:0315-162X
1499-2752
DOI:10.3899/jrheum.171248