Effect of Cyclosporine and Sirolimus on Fatty Acid Desaturase Activities in Cultured HEPG2 Cells

• Published in: Transplantation proceedings Vol. 41; no. 5; pp. 1865 - 1870
• Main Authors: Lausada, N, de Gómez Dumm, I.N.T, Raimondi, J.C, de Alaniz, M.J.T
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 01.06.2009
• Subjects: Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Arachidonic Acids - metabolism; Biological and medical sciences; Carcinoma, Hepatocellular; Cell Line, Tumor; Cyclosporine - pharmacology; Fatty Acid Desaturases - drug effects; Fatty Acid Desaturases - metabolism; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunosuppressive Agents - pharmacology; Kinetics; Linoleic Acid - metabolism; Liver Neoplasms; Medical sciences; Palmitic Acid - metabolism; Pharmacology. Drug treatments; Sirolimus - pharmacology; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology; Antineoplastic agent; Cell culture; Calcineurin inhibitor; Sirolimus; Enzyme; Lactone; Stearoyl-CoA desaturase; Transplantation; Macrolide; Biological activity; Macrocycle; Medicine; Antibiotic; Treatment; Surgery; Graft; Ciclosporin; Protein synthesis inhibitor; Oxidoreductases; Immunosuppressive agent; Antibacterial agent; Cell
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2009.03.063

Abstract The aim of the present work was to evaluate the influence of cyclosporine (CsA) and sirolimus (SRL) on fatty acid (FA) desaturase activities. These enzymes (named Δ9, Δ6, and Δ5 desaturases) catalyze reactions leading to the biosynthesis of n-9, n-6, and n-3 FA families. n-3 FA family, derived from alpha-linolenic acid, is involved in the prevention of vascular events, which appear after successful kidney transplantation. Five groups of HepG2 cells in culture were treated with either CsA (1 μg/μL and 2 μg/μL) or SRL (10 ng/mL and 20 ng/mL) for 3 days, including a control group without immunosuppressive treatment. We studied the incorporation and metabolic conversion of radioactive [1-14 C]palmitic, linoleic, and eicosatrienoic acids. We also analyzed fatty acid composition. The distribution of radioactive metabolic products after incubation of these cells with [1-14 C]palmitic acid revealed a decrease in Δ9 desaturase activity in the presence of each immunosuppressive drug: CsA = 0.61 ± 0.01; SRL = 0.59 ± 0.04 versus control = 0.79 ± 0.05 ( P < .01). We observed a significant increase in Δ6 and Δ5 desaturase activities under the influence of the immunosuppressive drugs: radiolabeled linoleic acid (CsA: 0.93 ± 0.04; SRL: 1.02 ± 0.03 vs control 0.60 ± 0.03; P < .01) and eicosatrienoic acid (CsA: 1.12 ± 0.02; SRL: 1.07 ± 0.01 vs control 0.75 ± 0.01; P < .01). In conclusion, CsA and SRL modulated the biosynthesis of polyunsaturated FAs, decreasing Δ9 desaturase and increasing Δ6 and Δ5 desaturase activities.